Switch off the unit, open the front panel, and carefully replace the lamp without touching the bulb with bare hands. B. Cleaning the Flow Cell
The Shimadzu SPD-20A is a high-performance UV-Vis detector that provides accurate and reliable detection of analytes in various samples. By following the operating instructions and troubleshooting tips outlined in this article, users can optimize the performance of the SPD-20A and achieve reliable results. Regular maintenance and repair are essential to ensure the longevity and optimal performance of the detector.
The is a high-performance, dual-wavelength UV/Vis detector designed for use with Shimadzu’s Prominence and LC-20A series liquid chromatography systems. This user manual serves as the definitive technical guide for laboratory personnel responsible for installation, operation, maintenance, and troubleshooting of the detector. shimadzu spd20a user manual
) solution, followed by a thorough flush with water until the pH returns to neutral. Never use hydrochloric acid. 5. Troubleshooting Common Issues Potential Cause Recommended Action Air bubble trapped in the flow cell.
The detector utilizes a deuterium lamp for UV light and an optional tungsten lamp (for AV model) for visible light. The optical path includes diffraction gratings and mirrors that must be kept clean to maintain sensitivity. Switch off the unit, open the front panel,
It offers a wide linearity of up to 2.5 AU, enabling accurate quantification across a broad concentration range.
(Interchangeable units):
Flush the cell with degassed methanol or isopropanol at a higher flow rate, or apply slight backpressure to the waste line manually using a syringe. Drastic ambient temperature changes or lamp warming up.
The is not a dusty binder to store on a shelf. It is an active diagnostic tool, a preventive maintenance schedule, and an educational resource. By familiarizing yourself with its structure—from the safety warnings in Chapter 1 to the error codes in the Appendix—you will reduce downtime, extend lamp life, and produce more reliable chromatographic data. This user manual serves as the definitive technical
The SPD-20A is typically operated via Shimadzu's software suite (such as LabSolutions or EZStart) through the CBM-20A communication module. Starting the Instrument
Turn off the detector and disconnect the power cord before replacing lamps or accessing the interior.