Eppendorf 5402 Manual

If samples are spilled, clean the rotor immediately. The F-45-18-11 rotor is susceptible to corrosion from aggressive chemicals.

Opposite tubes must contain equal fluid volumes. If you only have one sample tube, prepare a "balance tube" containing water of equal mass and volume.

Once the timer reaches zero, the centrifuge decelerates to a complete stop.

If you possess a physical copy of the , digitize it immediately. Scan every page at 300 DPI and store the PDF in a lab-shared drive (e.g., Google Drive or a local server). For everyone else, use the resources outlined in Part 2 to secure a copy. eppendorf 5402 manual

Check the lid gasket for cracks or wear to ensure proper sealing.

Regular maintenance ensures the centrifuge remains safe and accurate.

Adjustable from 1 to 99 minutes, or continuous operation. Dimensions: A tabletop unit requiring approximately cm of bench space. 2. Control Panel and Display If samples are spilled, clean the rotor immediately

Set the temperature dial to your desired target (e.g., 4°C).

: Always load the rotor symmetrically. If you have an odd number of samples, use a "balance tube" filled with an equal volume of water.

The Eppendorf 5402 is no longer in production. If you are considering a replacement for a faulty 5402, Eppendorf’s official successors are the and 5425 R refrigerated microcentrifuges. These newer models offer modern features like: If you only have one sample tube, prepare

The Eppendorf 5402 Refrigerated Microcentrifuge is a legendary piece of laboratory equipment, known for its robust construction, precise cooling capabilities, and reliability in molecular biology and biochemistry labs. Although it has been superseded by newer models, the 5402 remains a workhorse in many facilities.

Turn the device on and set the desired temperature using the cooling control button. Allow the unit to reach the temperature before starting the run. 3.2 Loading the Rotor Open Lid: Press the lid release button.

Pre-cools the rotor chamber rapidly before a run.

is designed to handle high-speed separations while maintaining strict climate control for your samples. Speed Range: Adjustable from 1,000 to 14,000 RPM , achieving a maximum RCF of 16,000 x g Temperature Control: Cooling range from -9°C to 40°C Standard rotor (F-45-18-11) holds of 1.5 mL to 2.0 mL. Fast Cooling: Features a

The technical capabilities of the Eppendorf 5402 dictate the types of protocols and tubes it can handle. Feature / Parameter Specification 14,000 RPM (Rotations Per Minute) Maximum RCF Approximately 16,000 × g (Relative Centrifugal Force) Temperature Range -9°C to +40°C Time Range 1 to 99 minutes, or continuous run mode Standard Rotor Capacity 18 or 24-place microcentrifuge tubes (1.5 mL / 2.0 mL) Power Consumption ~450 Watts 3. Control Panel Interface and Settings